Publications

2020    2019    2018    2017    2016    2015    2014    2013    2012

2020

Delbaere S, De Clercq A, Mizumoto S, Noborn F, Bek JW, Alluyn L, Gistelinck C, Syx D, Salmon PL, Coucke PJ, Larson G, Yamada S, Willaert A and Malfait F (2020) b3galt6 Knock-Out Zebrafish Recapitulate β3GalT6-Deficiency Disorders in Human and Reveal a Trisaccharide Proteoglycan Linkage Region. Front. Cell Dev. Biol. 8:597857. doi: 10.3389/fcell.2020.597857

Loontiens, S., Vanhauwaert, S., Depestel, L. et al. A novel TLX1-driven T-ALL zebrafish model: comparative genomic analysis with other leukemia models. Leukemia (2020). https://doi.org/10.1038/s41375-020-0938-2

Garcia, E.G., Veloso, A., Oliveira, M.L. et al. PRL3 enhances T-cell acute lymphoblastic leukemia growth through suppressing T-cell signaling pathways and apoptosis. Leukemia (2020). https://doi.org/10.1038/s41375-020-0937-3

2019

S. Delbaere, T. Van Damme, D. Syx, et al., Hypomorphic zebrafish models mimic the musculoskeletalphenotype ofb4GalT7-deficient Ehlers-Danlos syndr..., Matrix Biology,https://doi.org/10.1016/j.matbio.2019.12.002

Van Gils M, Vanakker OM. Morpholino-Mediated Gene Knockdown in Zebrafish: It Is All About Dosage and Validation. J Invest Dermatol. 2019 Jul;139(7):1599-1600.

Oralová V, Rosa JT, Soenens M, Bek JW, Willaert A, Witten PE, Huysseune A. Beyond the whole-mount phenotype: high-resolution imaging in fluorescence-based applications on zebrafish. Biol Open. 2019 May 24;8(5).

Loontiens S, Depestel L, Vanhauwaert S, Dewyn G, Gistelinck C, Verboom K, Van Loocke W, et al. Purification of High-Quality Rna from a Small Number of Fluorescence Activated Cell Sorted Zebrafish Cells for Rna Sequencing Purposes. BMC Genomics. 2019 Mar 20;20(1):228.

Cayuela ML, Claes KBM, Ferreira MG, Henriques CM, van Eeden F, Varga M,7, Vierstraete J, Mione MC. The Zebrafish as an Emerging Model to Study DNA Damage in Aging, Cancer and Other Diseases. Front Cell Dev Biol. 2019 Jan 10;6:178.

2018

Boel A, De Saffel H, Steyaert W, Callewaert B, De Paepe A, Coucke PJ, Willaert A. CRISPR/Cas9-mediated homology-directed repair by ssODNs in zebrafish induces complex mutational patterns resulting from genomic integration of repair-template fragments. Dis Model Mech. 2018 Oct 18;11(10).

Steyaert W, Boel A, Coucke P and Willaert A. BATCH-GE: Analysis of NGS Data for Genome Editing Assessment. Methods Mol Biol. 2018;1865:83-90. doi: 10.1007/978-1-4939-8784-9_6.

Gistelinck C, Kwon RY, Malfait F, Symoens S, Harris MP, Henke K, Hawkins MB, Fisher S, Sips P, Guillemyn B, Bek JW, Vermassen P, De Saffel H, Witten PE, Weis M, De Paepe A, Eyre DR, Willaert A*, Coucke PJ*. Zebrafish type I collagen mutants faithfully recapitulate human type I collagenopathies. Proc Natl Acad Sci U S A. 2018 Aug 21;115(34):E8037-E8046. *These authors jointly supervised this work.

Garcia EG, Iyer S, Garcia SP, Loontiens S, Sadreyev RI, Speleman F, Langenau DM. Cell of origin dictates aggression and stem cell number in acute lymphoblastic leukemia. Leukemia. 2018 Aug;32(8):1860-1865

2017

Van Gils M, Willaert A, De Vilder EYG, Coucke PJ, Vanakker OM. Generation and Validation of a Complete Knockout Model of abcc6a in Zebrafish. J Invest Dermatol. 2018 Nov;138(11):2333-2342. doi: 10.1016/j.jid.2018.06.183. Epub 2018 Jul 17.

Hur M, Gistelinck CA, Huber P, Lee J, Thompson MH, Monstad-Rios AT, Watson CJ, McMenamin SK, Willaert A, Parichy DM, Coucke P, Kwon RY. "microCT-based phenomics in the zebrafish skeleton reveals virtues of deep phenotyping in a distributed organ system. Zebrafish. 2017 Nov 27. doi: 10.1089/zeb.2017.1540.

Vierstraete J, Willaert A, Vermassen P, Coucke PJ, Vral A, Claes KBM. Accurate quantification of homologous recombination in zebrafish: brca2 deficiency as a paradigm. Sci Rep. 2017 Nov 28;7(1):16518.

Hur M, Gistelinck CA, Huber P, Lee J, Thompson MH, Monstad-Rios AT, Watson CJ, McMenamin SK, Willaert A, Parichy DM, Coucke P, Kwon RY. "microCT-based phenomics in the zebrafish skeleton reveals virtues of deep phenotyping in a distributed organ system. Elife. 2017 Sep 8;6. pii: e26014. doi: 10.7554/eLife.26014.

2016

Gistelinck C, Eckhard Witten P, Huysseune A, Symoens S, Malfait F, Larionova D, Simoens P, Dierick M, Van Hoorebeke L, De Paepe A, Kwon RY, Weis M, Eyre DR, Willaert A*, Coucke PJ*. Loss of Type I Collagen Telopeptide Lysyl Hydroxylation Causes Musculoskeletal Abnormalities in a Zebrafish Model of Bruck Syndrome. J Bone Miner Res. 2016 Aug 19. doi: 10.1002/jbmr.2977. *These authors jointly supervised this work.

Boel A, Steyaert W, De Rocker N, Menten B, Callewaert B, De Paepe A, Coucke P, Willaert A. BATCH-GE: Batch analysis of Next-Generation Sequencing data for genome editing assessment. Sci Rep. 2016 Jul 27;6:30330.

Vanhauwaert S, Lefever S, Coucke P, Speleman F, De Paepe A, Vandesompele J,Willaert A. RT-qPCR gene expression analysis in zebrafish: Preanalytical precautions and use of expressed repetitive elements for normalization. Methods Cell Biol. 2016;135:329-42. (IF:1,097)

C. Gistelinck*, R. Gioia*, A. Gagliardi, F. Tonelli, L. Marchese, L. Bianchi, C. Landi, L. Bini, A. Huysseune, P. E. Witten, A. Staes, K. Gevaert, N. De Rocker, B. Menten, F. Malfait, S. Leikin, S. Carra, R. Tenni, A. Rossi, A. De Paepe, P. Coucke, A. Willaert†, A. Forlino†. Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin. Sci Rep. 2016 Feb 15;6:21540). *These authors contributed equally to this work. *These authors jointly supervised this work.

2015

Sofie Symoens, Aileen M. Barnes, Charlotte Gistelinck, Fransiska Malfait, Brecht Guillemyn, Wouter Steyaert, Delfien Syx, Sanne D’hondt, Martine Biervliet, Julie De Backer, Eckhard P. Witten, Sergey Leikin, Elena Makareeva, Gabriele Gillessen-Kaesback, Ann Huysseune, Kris Vleminckx, Andy Willaert, Anne De Paepe, Joan C. Marini, Paul J. Coucke. Genetic defects in TAPT1 disrupt ciliogenesis and cause an in utero lethal osteochondrodysplasia. Am J Hum Genet. 2015 Oct 1;97(4):521-34.

Nina De Rocker, Sarah Vergult, Eva Jacobs, Susan Zeesman, Frédérique Béna, Nele Bockaert, Thomy de Ravel, Koenraad Devriendt, Sabrina Giglio, Laurence Faivre, Shelagh Joss, David Koolen, Saskia Maas, Ernie M Bongers, Francesca Novara, Malgorzata JM Nowaczyk, Hilde Peeters, Abeltje Polstra, Filip Roelens, Carla Rosenberg, Julien Thevenon, Zeynep Tümer, Suzanne Vanhauwaert, Konstantinos Varvagiannis, Andy Willaert, Marjolein Willemsen, Marjolaine Willems, Orsetta Zuffardi, Paul Coucke, Frank Speleman, Tjitske Kleefstra, Björn Menten. Refinement of the critical 2p25.3 deletion region: the role of MYT1L in intellectual disability and obesity. Genet Med. 2015 Jun;17(6):460-6.

2014

Suzanne Vanhauwaert, Gert Van Peer, Ali Rihani, Els Janssens, Pieter Rondou, Steve Lefever, Anne De Paepe, Paul J. Coucke, Frank Speleman, Jo Vandesompele, Andy Willaert. Expressed repeat elements improve RT-qPCR normalization across a wide range of zebrafish gene expression studies. PlosOne 2014 Oct 13;9(10):e109091

Vulto-van Silfhout AT, Rajamanickam S, Jensik PJ, Vergult S, de Rocker N, Newhall KJ, Raghavan R, Reardon SN, Jarrett K, McIntyre T, Bulinski J, Ownby SL, Huggenvik JI, McKnight GS, Rose GM, Cai X, Willaert A, Zweier C, Endele S, de Ligt J, van Bon BW, Lugtenberg D, de Vries PF, Veltman JA, van Bokhoven H, Brunner HG, Rauch A, de Brouwer AP, Carvill GL, Hoischen A, Mefford HC, Eichler EE, Vissers LE, Menten B, Collard MW, de Vries BB. Mutations Affecting the SAND Domain of DEAF1 Cause Intellectual Disability with Severe Speech Impairment and Behavioral Problems. Am J Hum Genet. 2014 May 1;94(5):649-61.

2013

Hosen MJ, Vanakker OM, Willaert A, Huysseune A, Coucke P, De Paepe A Zebrafish models for ectopic mineralization disorders: practical issues from morpholino design to post-injection observations. Front Genet. 2013 May 8;4:74.

2012

Willaert A,* Khatri S,* Callewaert B, Coucke P, Crosby S, Lee J, Davis E, Shiva S, Tsang M, De Paepe A, Urban Z. GLUT10 is required for the development of the cardiovascular system and the notochord and connects mitochondrial function to TGFβ signaling. Hum Mol Genet. 2012 15;21(6):1248-59. *These authors equally contributed to this work.