The facility holds a microscopy unit containing four Leica M80 stereomicroscopes, each provided with an Eppendorf Femtojet injector and WPI micromanipulator. These microscopes can be used for phenotyping, sorting and injection of zebrafish embryos/larvae. Furthermore, this unit contains a Leica M165FC and a Nikon SMZ18 fluorescence stereomicroscope provided with a sensitive Leica DF450C and DS-Qi camera respectively, suitable for both fluorescent and color imaging and two Zeiss Axio Observer inverted microscopes with automated time-lapse functionalities and 3D-deconvolution and apotome modules. For whole embryo confocal imaging, a Leica Super-zoom 3D confocal microscope is available.
The facility contains an immunohistochemical unit including a Micron HM355S Microtome with water bath and a Leica DM2000 microscope with camera to image zebrafish sections.
For rapid non-destructive genotyping of live zebrafish embryos, a Zebrafish Embryonic Genotyper (ZEG) device and sequencing unit is available.
Basic molecular techniques including morpholino-mediated gene knockdown, CRISPR/Cas mediated gene knock-out and knock-in, transgenesis using TOL2 and I-SceI constructs, whole mount in situ hybridization, qPCR and RNAseq-based gene-expression analysis and different (immuno)staining and imaging methods have been optimized and are frequently used in the facility.
Protocols are centrally archived and are therefore easily accessible for researchers.
The zebrafish facility, in its present form, has a capacity of about 17000 adult fish, which are housed in 6 “semi-closed” recirculating systems (ZebTEC and WTU systems, Tecniplast) that provide automatic water circulation, automatic monitoring and displaying of the most important water quality parameters such as temperature, pH and conductivity